A52 Development and evaluation of a viral-specific random PCR and next-generation sequencing based assay for detection and sequencing of hand, foot, and mouth disease pathogens
نویسندگان
چکیده
A50 The emergence of G8P[8] rotavirus group A across Vietnam Pham Thanh Duy,* Tran Thi Ngoc Dung,* October M. Sessions, Maia A. Rabaa, Stephen Baker, The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam, Program in Emerging Infectious Diseases Duke-National University of Singapore Graduate Medical School, Singapore, Singapore, Centre for Tropical Medicine Oxford University, Oxford, UK and The London School of Hygiene and Tropical Medicine, London, UK
منابع مشابه
Assessment of Foot and Mouth Virus Subtype O2016 Genetic Alterations During Successive Passages in BHK Monolayer
Abstract : Foot and Mouth Disease is one of the important live stocks contagious viral disease caused by Aphtovirus genus , that is belong to family RNA virus picornaviride. The important characteristic of FMD virus is high mutation that give rise to diversity of Antigen in surface of Neutralizing proteins. For this reason FMD virus have 7 distinct serotype and many subtype. Vaccination is o...
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Foot and mouth disease (FMD) is a contagious animal disease that causes irreparable damage to the economy of countries, including Iran in which this disease is a native one. Among the ways to combat FMD are vaccination and slaughter. Due to the specific situation of Iran, it is not possible to kill infected animals. Therefore, vaccination is the most important way to fight this disease. S...
متن کاملAn Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens.
OBJECTIVE To provide a feasible and cost-effective next-generation sequencing (NGS) method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples. METHODS Viruses from cell culture materials or cli...
متن کاملRapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples
During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV. The assay detected the viral RNA in all serotypes of FMDV. The rRT-PCR specifically detected FMD virus in sample with greater sensitivity than our convention...
متن کاملMolecular Testing in Microbiology
There are significant challenges associated with qualitative and quantitative nucleic acid tests performed in diagnostic laboratories. The development of internationally available certified reference materials which can be traced to reference measurements will contribute to a better understanding of the performance characteristics of nucleic acid tests and enhance reliability and comparability ...
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